Coding

Part:BBa_K3746002:Design

Designed by: Cheung Chi Ming   Group: iGEM21_Hong_Kong_JSS   (2021-10-17)


tvLac-6His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
    Illegal PstI site found at 1117
    Illegal PstI site found at 1160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
    Illegal PstI site found at 1117
    Illegal PstI site found at 1160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1515
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
    Illegal PstI site found at 1117
    Illegal PstI site found at 1160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
    Illegal PstI site found at 1117
    Illegal PstI site found at 1160
    Illegal AgeI site found at 99
    Illegal AgeI site found at 912
    Illegal AgeI site found at 1342
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a part design for the Phase I project (2021 iGEM). We plan to synthesize the gene fragment by IDT. A C-terminal 6-His tag was added for downstream purification purpose.

Source

The sequence of Trametes versicolor isolate K4 laccase CDS was extracted from NCBI (GenBank KR492189.1). Codon optimization for E. coli was done by Thermo Scientific GeneArt platform.


References